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woj wojtowicz rrid addgene 72065 pegfp n1 clontech n a  (Addgene inc)


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    Structured Review

    Addgene inc woj wojtowicz rrid addgene 72065 pegfp n1 clontech n a
    Woj Wojtowicz Rrid Addgene 72065 Pegfp N1 Clontech N A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1/Cntn1-Fc-His+(Plasmid+%2372065)/pm41861814-221-149-152
    Average 91 stars, based on 5 article reviews
    woj wojtowicz rrid addgene 72065 pegfp n1 clontech n a - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Phosphoinositide- and Collybistin-Dependent Synaptic Clustering of Gephyrin
    Article Snippet: .. Rat CB2 SH3- in mCherry-C3 described previously ( ) was subcloned into pQE80L (Qiagen) by PCR using the restriction sites BamHI and SalI and into pEGFP-N1 including a moxBFP tag (gift from Erik Snapp; Addgene plasmid # 68064; http://n2t.net/addgene:68064 ; RRID:Addgene_68064) via Gibson cloning. ..

    Plasmid Preparation:

    Article Title: Phosphoinositide- and Collybistin-Dependent Synaptic Clustering of Gephyrin
    Article Snippet: .. Rat CB2 SH3- in mCherry-C3 described previously ( ) was subcloned into pQE80L (Qiagen) by PCR using the restriction sites BamHI and SalI and into pEGFP-N1 including a moxBFP tag (gift from Erik Snapp; Addgene plasmid # 68064; http://n2t.net/addgene:68064 ; RRID:Addgene_68064) via Gibson cloning. ..

    Article Title: T cell protrusions enable fast, localised initiation of CAR signalling
    Article Snippet: For all plasmids harboring a SNAP-encoding sequence, the SNAP-tag sequence was taken from the pSNAPf (Addgene plasmid, #58186). .. For all plasmids harboring a GFP-encoding sequence, the GFP sequence was taken from pEGFP-N1 (Addgene plasmid, #6085-1). ..

    Article Title: Amyloid β–dependent neuronal silencing through synaptic decoupling
    Article Snippet: .. Used plasmids: pCMMP-TVA800 was a gift from Edward Callaway (Addgene plasmid # 15778); pAAV-EF1a-CVS-G-WPRE-pGHpA (Addgene plasmid # 67528) and pAAV-EF1a-tdTomato-WPRE-pGHpA (Addgene plasmid # 67527) were gifts from Botond Roska; pEGFP-N1 was bought from Addgene (Addgene plasmid # 6085-1). ..

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: .. The plasmid pEGFP-W (ref. 18), derived from pEGFP-N1 (Addgene, 6085-1), was purified from BL21(DE3) Escherichia coli (NEB, C2527H) using a Qiagen plasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. .. Specifically, 20 U of each enzyme was added to 5 μg of the plasmid along with 5 μl of 10× rCutsmart buffer (NEB, B6004S) in a final volume of 50 μl, followed by incubation for 1 h at 37 °C.

    Article Title: Supporting Information for Stearoylation cycle regulates the cell surface distribution of the PCP protein Vangl2
    Article Snippet: .. The cDNA of human Vangl2 was obtained from UltimateTM Human ORF Clones (Invitrogen), and was subcloned into pCMV-3xFlag, pEGFP-N1, pWPXL (A gift from Didier Trono’s lab, Addgene plasmid # 12257), and pLenti CMV rtTA3 Hygro (w785-1) (A gift from Eric Campeau’s lab, Addgene plasmid # 26730) vectors. .. The cDNAs of human ZDHHCs, APT1/2, LAMP2A and YAP were obtained from UltimateTM Human ORF Clones (Invitrogen) or synthesized from RNA by reverse transcription, and were subcloned into pCDNA3.1-HA or pCMV-3xFlag vector.

    Article Title: Amyloid β-dependent neuronal silencing through synaptic decoupling.
    Article Snippet: .. Used plasmids: pCMMP- TVA800 was a gift from Edward Callaway (Addgene plasmid # 15778); pAAV- EF1a- CVS- G- WPRE- pGHpA (Addgene plasmid # 67528) and pAAV- EF1atdTomato- WPRE- pGHpA (Addgene plasmid # 67527) were gifts from Botond Roska; pEGFP- N1 was bought from Addgene (Addgene plasmid # 6085- 1). ..

    Cloning:

    Article Title: Phosphoinositide- and Collybistin-Dependent Synaptic Clustering of Gephyrin
    Article Snippet: .. Rat CB2 SH3- in mCherry-C3 described previously ( ) was subcloned into pQE80L (Qiagen) by PCR using the restriction sites BamHI and SalI and into pEGFP-N1 including a moxBFP tag (gift from Erik Snapp; Addgene plasmid # 68064; http://n2t.net/addgene:68064 ; RRID:Addgene_68064) via Gibson cloning. ..

    Sequencing:

    Article Title: T cell protrusions enable fast, localised initiation of CAR signalling
    Article Snippet: For all plasmids harboring a SNAP-encoding sequence, the SNAP-tag sequence was taken from the pSNAPf (Addgene plasmid, #58186). .. For all plasmids harboring a GFP-encoding sequence, the GFP sequence was taken from pEGFP-N1 (Addgene plasmid, #6085-1). ..

    Derivative Assay:

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: .. The plasmid pEGFP-W (ref. 18), derived from pEGFP-N1 (Addgene, 6085-1), was purified from BL21(DE3) Escherichia coli (NEB, C2527H) using a Qiagen plasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. .. Specifically, 20 U of each enzyme was added to 5 μg of the plasmid along with 5 μl of 10× rCutsmart buffer (NEB, B6004S) in a final volume of 50 μl, followed by incubation for 1 h at 37 °C.

    Purification:

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: .. The plasmid pEGFP-W (ref. 18), derived from pEGFP-N1 (Addgene, 6085-1), was purified from BL21(DE3) Escherichia coli (NEB, C2527H) using a Qiagen plasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. .. Specifically, 20 U of each enzyme was added to 5 μg of the plasmid along with 5 μl of 10× rCutsmart buffer (NEB, B6004S) in a final volume of 50 μl, followed by incubation for 1 h at 37 °C.

    Ligation:

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: .. The plasmid pEGFP-W (ref. 18), derived from pEGFP-N1 (Addgene, 6085-1), was purified from BL21(DE3) Escherichia coli (NEB, C2527H) using a Qiagen plasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. .. Specifically, 20 U of each enzyme was added to 5 μg of the plasmid along with 5 μl of 10× rCutsmart buffer (NEB, B6004S) in a final volume of 50 μl, followed by incubation for 1 h at 37 °C.

    Clone Assay:

    Article Title: Supporting Information for Stearoylation cycle regulates the cell surface distribution of the PCP protein Vangl2
    Article Snippet: .. The cDNA of human Vangl2 was obtained from UltimateTM Human ORF Clones (Invitrogen), and was subcloned into pCMV-3xFlag, pEGFP-N1, pWPXL (A gift from Didier Trono’s lab, Addgene plasmid # 12257), and pLenti CMV rtTA3 Hygro (w785-1) (A gift from Eric Campeau’s lab, Addgene plasmid # 26730) vectors. .. The cDNAs of human ZDHHCs, APT1/2, LAMP2A and YAP were obtained from UltimateTM Human ORF Clones (Invitrogen) or synthesized from RNA by reverse transcription, and were subcloned into pCDNA3.1-HA or pCMV-3xFlag vector.

    other:




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